Reproducibility of Circulating Endothelial Cell Enumeration and Activation in Children and Adolescents
Year: 2016
Type: article
Study design: Observational Study
Abstract: INTRODUCTION: We examined the reproducibility of circulating endothelial cells (CEC) enumeration and activation among youth. MATERIALS AND METHODS: CECs from 151 youth were measured at baseline and 1 week follow-up. Enumeration of CEC in fresh whole blood was determined by direct assessment of buffy coat smears (CD146+ nucleated cells) and activated CEC (%VCAM-1 expression) was determined after immunomagnetic enrichment and co-staining of nuclei, plus positivity for P1H12 and VCAM-1. RESULTS: No statistically significant difference in CEC enumeration (1.2 ± 2.5 vs 1.3 ± 2.2 CEC/milliliter of whole blood, p = 0.745) or activated CEC (57.1 ± 24.4 vs 58.0 ± 21.3 %VCAM-1, p = 0.592) between baseline and 1 week follow-up. CONCLUSION: On a cohort basis, CEC enumeration and activation are reproducible in youth. Relatively high individual biological variability may limit its clinical utility.
Source: Biomarkers in Medicine
Authors: Justin R Ryder, Michael J O’Connell, Kyle D Rudser, Claudia K Fox, Anna N Solovey, Robert P Hebbel, Aaron S Kelly
Institution: University of Minnesota
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2340327599