Work

Specific quantification of mouse and human keratin proteins by radioimmunoassay

Year: 1980

Type: article

Abstract: Mouse epidermal keratin proteins were purified and labelled with 125I by chemical techniques. A radioimmunoassay method was established with a rabbit antibody elicited against the mouse keratin. This assay method was utilized to quantify keratin proteins of mouse and human epidermal extracts, both from intact tissues and cells in culture. As little as 30 ng of mouse keratin (0.5 pmol) was quantifiable.

Source: Biochemical Journal

DOI: 10.1042/bj1870281

Authors: S H Yuspa, M C Poirier, J R Harness, D R Olsom, P M Steinert

Institutions: National Institutes of Health, National Cancer Institute

Language: English

Topic: Skin and Cellular Biology Research

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W2344412358

FWCI: 1.106

Cites: 21

Cited by: 13

Related to: 10