Functional investigation of arabidopsis long coiled-coil proteins and subcellular localization of plant rangap1
Year: 2004
Type: article
Abstract: Long alpha-helical coiled-coil proteins are involved in a variety of organizational and regulatory processes in eukaryotic cells.In contrast to yeast and animals, only few such proteins have been investigated in plants.Here, two plant long coiled-coil proteins were investigated in detail, MAR-binding filament-like protein1 (MFP1) and the putative Arabidopsis homolog of Tpr (translocated promoter region).MFP1 is a nuclear-encoded, long coiled-coil protein that is targeted to plastids.It accumulates to comparable levels in all tissues of Arabidopsis which contain green chloroplasts, regardless of age and organ identity, but is much less abundant in roots of both light-grown and dark-grown seedlings.MFP1 protein accumulation parallels chloroplast development during the greening of seedlings shifted from dark to light, suggesting that MFP1 expression is regulated in a tissue-specific and light-dependent manner.MFP1 is localized in chloroplasts both in suspension culture cells and in leaves, and it is associated with the stromal side of thylakoid membranes of mature chloroplasts.It is co-purified with nucleoids, suggesting a function at the interface of the chloroplast genome and the photosynthetic membranes.MFP1 I deeply appreciate my adviser,
Author: Sun Yong Jeong
Institution: The Ohio State University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2346615954