Year: 2016
Type: article
Abstract: Second harmonic generation (SHG) imaging can be used to visualize unique biological phenomena, but currently available dyes limit its application owing to the strong fluorescent signals that they generate together with SHG. Here we report the first non-fluorescent and membrane potential-sensitive SHG-active organic dye Ap3. Ap3 is photostable and generates SH signals at the plasma membrane with virtually no fluorescent signals, in sharp contrast to the previously used fluorescent dye FM4-64. When tested in neurons, Ap3-SHG shows linear membrane potential sensitivity and fast responses to action potentials, and also shows significantly reduced photodamage compared with FM4-64. The SHG-specific nature of Ap3 allows simultaneous and completely independent imaging of SHG signals and fluorescent signals from various reporter molecules, including markers of cellular organelles and intracellular calcium. Therefore, this SHG-specific dye enables true multimodal two-photon imaging in biological samples.
Source: Nature Communications
DOI: 10.1038/ncomms11557
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $6,990
Awards: Development of caged compounds via photoinduced electron transfer reaction, Development of non-luminescent molecules for multimodal two-photon imaging, Visualization of biological phenomena by the second harmonic generation imaging using the next generation dye., G7829436476, Development of non-fluorescent imaging technique for biological application, Characterization of neuronal physiology by second harmonic generation imaging
OpenAlex ID: W2346937832