Work

Selection and optimization for lipid production of a newly isolated oleaginous yeast, rhodosporidium toruloides DMKU3-TK16

Year: 2010

Type: article

Abstract: Rhodosporidium toruloides DMKU3-TK16, an oleaginous yeast, was isolated from soil by an enrichment technique in nitrogen-limited medium I. Lipid production by this strain was highest in shaking flask cultivation at 150 rpm and 28°C in nitrogen-limited medium II containing per liter 70 g glucose, 0.75 g yeast extract, 0.55 g (NH4)2SO4, 0.4 g KH2PO4, 2.0 g MgSO4•7H2O, with a pH of 5.5. Under these optimal conditions, when the C/N ratio of the medium was 140, R. toruloides DMKU3-TK16 produced a lipid quantity of 9.26 g/L, which was 71.30 % of the dry biomass (13.33 g/L) after 168 h ofcultivation. The major fatty acids of the cellular lipid were oleic acid (41.54%), palmitic acid (22.49%), linoleic acid (15.12%) and steric acid (14.56%).

Source: Agriculture and Natural Resources

Authors: Pakawat Kraisintu, Wichien Yongmanitchai, Savitree Limtong

Institution: Kasetsart University

Language: English

Topic: Microbial Metabolic Engineering and Bioproduction

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDGs: Responsible consumption and production, Zero hunger

Open Access status: diamond

Funders: Thailand Research Fund, Kasetsart University

OpenAlex ID: W234759217

FWCI: 0.743

Cites: 23

Cited by: 61

Related to: 10