Improvement of wheat abiotic stress resistance via genetic transformation
Year: 2002
Type: article
Abstract: Aldose reductase gene (ALR) isolated from alfalfa (MsALR), was constructed into a plant expression vector (pAHC), and then named as pAHALR. Wheat suspension culture was bombarded with this plasmid and the transformed calli were cultured and selected on medium containing 10mg/L Bialaphos (15% glufozinate-ammonium) as a selective agent. For the molecular analysis of putative transgenic samples, PCR, Southern and Western blots were carried out. Also aldose reductase enzyme activity was detected by HPLC only in the transgenic callus samples by the accumulation of sorbitol, which is the end product of aldose reductase catalyzed glucose reduction reaction. Protective function of ALR was verified by malondi- aldehyde analysis under stress condition. The activity of the ALR gene will also be investigated on the transgeneic plants by biochemical and physiological analysis. Acta Biol Szeged 46(3-4):5-7 (2002)
Source: Acta Biologica Szegediensis
Institutions: University of Szeged, Hungarian Academy of Sciences
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W2350684343