Work

Subcellular localization and quantification of cholesterol in cultured human fibroblasts exposed to human low density lipoprotein

Year: 1982

Type: article

Abstract: Subcellular localization of nonesterified cholesterol has been determined in normal human fibroblasts from cultures incubated with human low density lipoprotein (LDL). Nonesterified and esterified cholesterol content of fibroblasts, grown initially in the absence of cholesterol, increased significantly after a 1-hour incubation with LDL. Digitonin was used to localize nonesterified cholesterol that was accumulated within multivesicular and lamellar lysosomal inclusions. This was observed only in fibroblasts from cultures incubated with LDL. Accumulation of LDL-derived nonesterified cholesterol within lysosomes is consistent with the suggestion of other investigators that LDL is metabolized within lysosomes.

Source: Journal of Lipid Research

DOI: 10.1016/s0022-2275(20)38049-4

Authors: H S Kruth, J Blanchette-Mackie, J Avigan, W Gamble, M Vaughan

Institutions: National Institutes of Health, Oregon State University, Uniformed Services University of the Health Sciences, National Heart, Lung, and Blood Institute

Language: English

Topic: Cholesterol and Lipid Metabolism

Subfield: Surgery

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,430