Experimental study of the transmission of porcine endogenous retrovirus in treatment of bioartificial liver system for acute liver failure canines
Year: 2007
Type: article
Abstract: Objective: To study the transmission of porcine endogenous retrovirus after perfusion in vitro and treatment for acute liver failure canines using bioartificial liver system based on porcine hepatocytes. Methods:The novel bioartificial liver system (BAL) was constructed. The hepatocyte suspensions were circulated in BAL system for 6h. ALF was induced by end-side portocaval shunt combined with common bile duct ligation and transection. BAL was used to treat acute liver failure canines for 6h. PCR was used for detection of PERV of porcine hepaocytes. RT-PCR was used for detection of PERV RNA of circulated liquid in the hollow fiber calibers and serum of canines pre-circulation to post-circulation. Results:The results showed that PERV was detectable in porcine hepaocytes and PERV RNA was not detectable in the circulated liquid and sera of the canines pre-circulation and post-circulation. Conclusion:The spread of PERV had not been found after circulation of 6h in vitro and treatment of 6h for ALF canines using the novel BAL system based on porcine hepatocyte.
Author: Yitao Ding
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $50
OpenAlex ID: W2355876746