A new assay for soluble transferrin receptors in serum: time for standardisation.
Year: 1997
Type: letter
Abstract: Sir, In a previous issue of this Journal we described the clinical and analytical implications of two recently introduced assays for soluble transferrin receptors in serum (1). Recently we were able to perform a short study on the Orion transferrin receptor assay (Orion Espoo, Finland) which is being introduced to the market now. Apart from an analysis of the analytical performances of the assay we also carried out a limited clinical study on patient samples to compare the clinical usefullness of the Orion assay with the Ramco assay (Ramco, Houston, TX, USA). To determine the intra-assay imprecision 10 replicate measurements with 8 samples were performed. The mean soluble transferrin receptors concentration ranged from 0.6—9.0 mg/1. The intraassay imprecision ranged from 3.9% to 6.1%. Duplicate measurements of 2 samples were performed in order to determine the interassay imprecision. Each sample was analysed in two different assays at the same day. Additionally, the end-of-run drift was determined by analysing the same 2 samples in the first wells as well as in the last wells of the microplate in two runs. The inter-assay imprecision was 4.0% and 6.2% and no end-of-run drift was found. [...]
Source: PubMed
Authors: E P Kuiper-Kramer, J van Raan, H G van Eijk
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2364003843