Fatty acids liberated from lipoproteins by lipoprotein lipase negatively influences cholesterol efflux from THP-1 macrophages
Year: 2014
Type: dissertation
Abstract: Lipoprotein lipase (LPL) is an extracellular lipase that primarily hydrolyses \ntriglycerides within circulating lipoproteins. Macrophage LPL contributes to \natherogenesis, but the mechanisms behind it are poorly understood. I hypothesized that \nthe free fatty acid (FFA) component of the products of lipoprotein hydrolysis generated \nby LPL promotes atherogenesis by inhibiting the cholesterol efflux ability by \nmacrophages. To test my hypothesis, THP-1 macrophages were incubated overnight with \nlipoprotein hydrolysis products generated by LPL. Results showed that the hydrolysis \nproducts negatively modulated the transcripts encoding nuclear receptors, cholesterol \ntransporters, and enzymes involved in FFA synthesis. A mixture of only purified FFA that \nmatches those liberated by LPL yielded comparable results to those for lipoprotein \nhydrolysis products. Furthermore, the FFA mixture significantly attenuated \napolipoprotein A-I-mediated cholesterol efflux. Overall, these data show that lipoprotein \nhydrolysis products generated by LPL may promote atherogenesis by inhibiting \ncholesterol efflux, which partially explains the pro-atherogenic role of macrophage LPL.
Author: Yanbo Yang
Language: English
Subfield: Biochemistry
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W237106115