Ion-beam-mutation breeding of an arachidonic acid biosynthesis microorganism and its industrial fermentation control
Year: 2012
Type: article
Abstract: Some microorganisms in nature produce high-quality oils. These oils provide nutritional benefits to human health, and can be potential sources of biodiesel. Developing microorganism-derived oils, also known as single-cell oils, is particularly important for China because of the shortage of oil resources. We have bred a high-yielding, polyunsaturated fatty acids (PUFCs)-producing strain from the fungi Mortierella alpine by using a two-step strategy of ion-beam-mutation breeding and PUFCs productivity screening. We further studied the nutritional requirements of this new strain, and developed a technique that recycles the fungi residues, after oil extraction, into fermentation substrates. A biomass of 38.2 g/L, 20.67 g/L of which were oils, was reached in the 6×50 m3 and 4×200 m3 highcapacity reactors, designed for mycelial fungus fermentation at high-density, with optimized energy and mass transmission efficiency. Among the oils, the content of the physiologically active arachidonic acid reached 9.89 g/L at the highest level and 8.97 g/L on average. Residual oils, after arachidonic acid extraction and refinement, were processed into biodiesel, the main indexes of which attained the national standards.
DOI: 10.1360/972011-2199
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W2374020654