Work

Organelle-Targeted H2S Probes Enable Visualization of the Subcellular Distribution of H2S Donors

Year: 2016

Type: article

Abstract: Hydrogen sulfide (H2S) is an essential biological signaling molecule in diverse biological regulatory pathways. To provide new chemical tools for H2S imaging, we report here a fluorescent H2S detection platform (HSN2-BG) that is compatible with subcellular localization SNAP-tag fusion protein methodologies and use appropriate fusion protein constructs to demonstrate mitochondrial and lysosomal localization. We also demonstrate the efficacy of this detection platform to image endogenous H2S in Chinese hamster ovary (CHO) cells and use the developed constructs to report on the subcellular H2S distributions provided by common H2S donor molecules AP39, ADT-OH, GYY4137, and diallyltrisulfide (DATS). The developed constructs provide a platform poised to provide new insights into the subcellular distribution of common H2S donors and a useful tool for investigating H2S biochemistry.

Source: Analytical Chemistry

DOI: 10.1021/acs.analchem.6b00087

Authors: Leticia A. Montoya, Michael D. Pluth

Institution: University of Oregon

Language: English

Topic: Sulfur Compounds in Biology

Subfield: Biochemistry

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Alfred P. Sloan Foundation, National Institute of General Medical Sciences

Award: Hydrogen Sulfide and Carbonyl Sulfide Delivery for Biological Applications