Experimental of Combining Therapy with IL-12 Gene and THP in Bladder Cancer Nude Mice Model
Year: 2010
Type: article
Abstract: Objective To explore the inhibition of mouse interleukin-12(IL-12) gene and THP on bladder cancer nude mice model.Methods After subcutaneous injection of EJ human bladder tumor cells to the nude mice subaxillary,Nude mice were divided into four groups randomly(Combine,IL-12,THP and control groups).Nude mice in Combine group were injected plasmid pCAGGS-mIL-12 on skeletal muscle and THP peritoneally.IL-12 group only were injected plasmid pCAGGS-mIL-12 on skeletal muscle;THP group were injected THP peritoneally;Control group were injected saline peritoneally and on skeletal muscle.All injections were carried two times per week for two weeks.The expression of IL-12 and IFN-γin blood was assayed by an enzyme-linked immunosorbent assay(ELISA) at one week after injection.Mice were sacrificed at fourth week.The level of VEGF in tumor tissue was detected by immunohistochemistry.IL-12 and IFN-γin tumor tissue was assayed by ELISA.Results In combine group,tumor growth were retarded compared with the other groups.There was a significantly low expression of VEGF in the combined group;IL-12 and IFN-γwere increased in both the IL-12 group and combined group.Conclusion Both injected pCAGGS-mIL-12 on skeletal muscle and THP peritoneally could inhibit the xenograft in nude mice planted with human bladder tumor cells.
Source: Zhongliu fangzhi yanjiu
Author: Fengqi Wang
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $72
OpenAlex ID: W2381631706