Work

Peroxidase Overproduction in Tomato: Wound-induced Polyphenol Deposition and Disease Resistance

Year: 1993

Type: article

Abstract: Lignin composition in leaf, fruit, and fruit outer epidermis of transgenic tomato (Lycopersicon esculentum Mill.) plants that overproduce the enzyme tobacco anionic peroxidase (TobAnPOD) was analyzed. This enzyme may catalyze the polymerization of cinnamyl alcohols into lignin in tobacco (Nicotiana tabacum L.); therefore, we predicted that its presence in the transformed tissue would increase lignin levels in healthy and wounded tissue. Lignin levels in healthy plants increased by 20% in leaf, 49% in fruit, and 106% in fruit outer epidermal tissue. Mature-green fruit were aseptically wounded and incubated in darkness for up to 7 days. Soluble phenols in wounded transgenic fruit increased by more than 300% hut changed little in control fruit. As with soluble phenols, lignin content in wounded transformed fruit increased by more than 20-fold hut increased less than two-fold in control fruit. Transgenic seedlings overproducing TobAnPOD were screened for susceptibility to several pathogens, but resistance did not increase. Possible TobAnPOD roles in lignin biosynthesis, phenol metabolism, stress response, and disease resistance are discussed.

Source: HortScience

DOI: 10.21273/hortsci.28.3.218

Authors: L. Mark Lagrimini, Jill Vaughn, W. Alan Erb, Sally A. Miller

Institution: The Ohio State University

Language: English

Topic: Plant Gene Expression Analysis

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: bronze

Funder: Ohio State University