Detection of Micrometastasis and Its Clinical Significance in Lymph Nodes from N0 Colorectal Cancers by FQ-PCR
Year: 2008
Type: article
Study design: Observational Study
Abstract: Objective To study the detection of lymph node micrometastasis in N0 colorectal cancer patients and its clinical significance in these patients.Methods In the study,453 lymph nodes obtained from 45cases of N0 colorectal cancer going curative operation were examined,using fluorescent quantity polymerase chain reaction(FQ-PCR) assay to detect cytokeratin 20(CK20) mRNA expression for identifying micrometastasis.Results Micrometastasis was detected in 46 lymph nodes(10.2%,46/453) of 20 cases(44.4%,20/45).The presence of micrometastasis was correlated with invasion depth of primary tumor,but was not related to sex,age,size and differentiation of primary tumor.The mean follow-up time in the positive groups(20 cases) with micrometastasis and the negative groups(25 cases) without micrometastasis were 19.6 and 22.4 months respectively,but there were 7 cases post-operative recurrence,metastases and 5 cases died in positive groups(20 cases) with micrometastasis,only 1 case died because of recurrence or metastases in negative groups(25 cases) without micrometastasis(χ2=7.305,P0.05).The disease-free survival of positive and negative groups was 75.0%(15/20) and 96.0%(24/25),respectively.The two groups have significant difference(χ2=4.240,P0.05).Conclusion CK20 FQ-PCR assay is a sensitive and specific method to detect lymph node micrometastasis in the patients with N0 colorectal cancer.The detection of CK20 mRNA in lymph nodes may contribute to improve the accuracy of clinical staging,determine patients' prognosis and provide information for rational adjuvant therapy.
Source: Zhongliu fangzhi yanjiu
Author: Lei Zhu
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $72
OpenAlex ID: W2388860121