Induction of immunoglobulin and antigen-dependent antibody synthesis in human lymphocytes, using supernatants from mitogen-stimulated cultures.
Year: 1983
Type: article
Abstract: Supernatants derived from pokeweed mitogen-stimulated peripheral blood lymphocytes were freed of lectin by passage over an antibody column: the eluate contained <2 ng/ml of PWM, measured by radioimmunoassay. In serum-free medium, these supernatants induce B-cell but not T-cell proliferation. Maximum proliferation is seen only if the B cells are co-cultured with irradiated T cells. The supernatants induce polyclonal immunoglobulin production, and in the presence of a soluble protein antigen (tetanus toxoid) they also induce specific antibody synthesis, in donors immunized up to 18 months previously.
Source: PubMed
Authors: M E North, M K Brenner
Institutions: Medical Research Council, Houston Methodist
Language: English
Topic: T-cell and B-cell Immunology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2395092417