Work

Recombinant E. coli Prototype Strains for in Vivo Glycorandomization

Year: 2010

Type: article

Abstract: In vitro glycorandomization is a powerful strategy to alter the glycosylation patterns of natural products and small molecule therapeutics. Yet, such in vitro methods are often difficult to scale and can be costly given the requirement to provide various nucleotides and cofactors. Here, we report the construction of several recombinant E. coli prototype strains that allow the facile production of a range of small molecule glycosides. This strategy relies on the engineered promiscuity of three key enzymes, an anomeric kinase, a sugar-1-phosphate nucleotidyltransferase, and a glycosyltransferase, as well as the ability of diverse small molecules to freely enter E. coli. Subsequently, this work is the first demonstration of "in vivo glycorandomization" and offers vast combinatorial potential by simple fermentation.

Source: ACS Chemical Biology

DOI: 10.1021/cb100267k

Authors: Gavin J. Williams, Jie Yang, Changsheng Zhang, Jon S. Thorson

Institution: University of Wisconsin–Madison

Language: English

Topic: Carbohydrate Chemistry and Synthesis

Subfield: Organic Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funder: National Institute of Allergy and Infectious Diseases

Awards: G1956070232, In Vitro Glycorandomization of Natural Products, In Vitro Glycorandomization of Natural Products