The biosynthesis of dentine γ-carboxyglutamic acid-containing proteins by rat incisor odontoblasts in organ culture
Year: 1983
Type: article
Abstract: Rat odontoblasts were shown to synthesize and secrete gamma-carboxyglutamic acid(Gla)-containing proteins into dentine after organ culture in the presence of radiolabelled amino acid precursors. Purified dentine Gla-containing protein from rat incisors was used as antigen to prepare rabbit antisera as a probe of dentine Gla-containing-protein biosynthesis in organ cultures of dentine (rat incisor) and bone (rat calvaria). Use of the antiserum also pointed out the cross-reactivity of a high-M, glycoprotein present within the dentine matrix. The present results are significant in identifying dentine gla-containing protein as endogenous to mineralizing dentine and may relate to the commonality between calcifying connective tissues in general.
Source: Biochemical Journal
DOI: 10.1042/bj2160249
Authors: M T DiMuzio, M Bhown, W T Butler
Institution: University of Alabama at Birmingham
Language: English
Subfield: Rheumatology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W2396961431