Work

Alignment, classification, and three-dimensional reconstruction of single particles embedded in ice.

Year: 1992

Type: article

Abstract: Cryo-electron microscopy of single biological particles poses new challenges to digital image processing due to the low signal-to-noise ratio of the data. New tools have been devised to deal with important aspects of 3-D reconstruction following the random-conical data collection scheme: (a) a new shift-invariant function has been derived, which promises to facilitate alignment and classification of single particle projections; (b) a new method of orientation search is proposed, which makes it possible to relate random-conical data sets to one another prior to reconstruction; and (c) the foundation is laid for a 3-D variance estimation which utilizes the oversampling of 3-D angular space by projections in the random-conical reconstruction scheme.

Source: PubMed

Authors: J Frank, P Penczek, W Liu

Institutions: New York State Department of Health, Wadsworth Center

Language: English

Topic: Advanced Electron Microscopy Techniques and Applications

Subfield: Structural Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Science Foundation, National Institutes of Health, National Institute of General Medical Sciences

Awards: Computer for Ultrastructure Research and Molecular Biology, Structural Analysis of Macromolecular Assemblies, COMPUTER FOR ULTRASTRUCTURE RESEARCH &MOLECULAR BIOLOGY

OpenAlex ID: W2397238275

FWCI:

Cites:

Cited by: 8

Related to: 10