Year: 1961
Type: article
Abstract: Although elementary statistical methods such as the calculation of means and their standard errors are commonly employed, application of the statistical methods of regression analysis in enzyme kinetic studies has received little attention.In estimating the kinetic parameters of the Michaelis-Menten equation, for instance, graphical methods such as the double-reciprocal plot (Lineweaver & Burk, 1934) are generally used, without supple- mentary statistical calculations.These methods have been reviewed by Dixon & Webb (1958).Graphical methods, however, do not usually provide any measure of the precision of the deter- minations, a knowledge of which is necessary for a proper evaluation of the results in relation to theoretical considerations, or for a comparison of results obtained under differing experimental conditions.The main purpose of a statistical analysis is to supply, not only more accurate estimates, but also the necessary measures of
Source: Biochemical Journal
DOI: 10.1042/bj0800324
Author: GN WILKINSON
Institution: The University of Adelaide
Language: English
Subfield: Applied Mathematics
Field: Mathematics
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W2397861637