Work

Polymorphisms in TIM-3 and breast cancer susceptibility in Chinese women: A case-control study

Year: 2016

Type: article

Abstract: // Zheng Wang 1, 2 , Xinghan Liu 1 , Xijing Wang 1 , Tie Chong 3 , Shuai Lin 1 , Meng Wang 1 , Xiaobin Ma 1 , Kang Liu 1 , Peng Xu 1 , Yanjing Feng 1 , Zhijun Dai 1 1 Department of Oncology, Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004, China 2 Department of Medical Oncology, Xi’an Central Hospital, Xi’an 710004, China 3 Department of Urologic Surgery, Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004, China Correspondence to: Zhijun Dai, email: [email protected] ; [email protected] Keywords: TIM-3, breast cancer, polymorphism, case-control study Received: December 22, 2015 Accepted: May 12, 2016 Published: May 27, 2016 ABSTRACT Previous studies have found associations between polymorphisms in T cell immunoglobulin and mucin domain 3 ( TIM-3 ) and increased risks of various cancers. However, the association between TIM-3 polymorphisms and breast cancer (BC) remains uncertain. In this study, a total of 560 BC patients and 583 age, sex, and ethnicity-matched healthy controls from Northwest China were included. The polymorphisms were genotyped using Sequenom MassARRAY. The expression level of TIM-3 protein was detected by immunohistochemistry. We observed rs10053538 had a significantly increased risk of BC, comparing with the wild-type genotype even after Bonferroni correction. In addition, the rs4704853 G>A variants were more frequent among BC patients than the controls (GA + AA vs. GG: OR = 1.32, 95% CI = 1.03-1.69, P = 0.026); However, the significance was lost after Bonferroni correction (P = 0.078). Furthermore, rs10053538 was associated with lymph node metastasis. Age stratification revealed that among patients aged <49 years, those with the rs4704853 GA/AA genotype had a higher risk of BC; But there was no difference when Bonferroni correction was conducted. Immunohistochemical analysis showed that the expression of TIM-3 protein in the breast cancer tissues was higher in patients carrying the rs10053538 GT+TT genotype than those with GG genotype (P = 0.012). However, we failed to find any difference between BC patients and controls in any rs1036199 genetic model. These findings suggested that rs10053538 in TIM-3 might increase susceptibility to BC and promote the progression of BC in Chinese women.

Source: Oncotarget

DOI: 10.18632/oncotarget.9665