Work

A Rapid Method for Refolding Cell Surface Receptors and Ligands

Year: 2016

Type: article

Abstract: Production of membrane-associated cell surface receptors and their ligands is often a cumbersome, expensive, and time-consuming process that limits detailed structural and functional characterization of this important class of proteins. Here we report a rapid method for refolding inclusion-body-based, recombinant cell surface receptors and ligands in one day, a speed equivalent to that of soluble protein production. This method efficiently couples modular on-column immobilized metal ion affinity purification and solid-phase protein refolding. We demonstrated the general utility of this method for producing multiple functionally active immunoreceptors, ligands, and viral decoys, including challenging cell surface proteins that cannot be produced using typical dialysis- or dilution-based refolding approaches.

Source: Scientific Reports

DOI: 10.1038/srep26482

Authors: Lu Zhai, Ling Wu, Feng Li, Robert S. Burnham, Juan C. Pizarro, Bin Xu

Institutions: Virginia Tech, Tulane University

Language: English

Topic: Protein purification and stability

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,445

Funders: U.S. Department of Agriculture, Diabetes Action Research and Education Foundation, Strong, National Institute of Food and Agriculture