Work

Studies on Connexin 43, a Gap-Junction Protein, in P19 Embryonal Carcinoma Cells after Culture on an Apatite Fiber Scaffold

Year: 2016

Type: article

Abstract: We previously showed enhanced osteoblast differentiation by culturing cells in apatite-fiber scaffold (AFS). The well-developed a-surface of the apatite fibers provides favorable structural features and chemical interactions with the cells, and the scaffold appears to affect cell differentiation. AFS was used here to study its utility for soft-tissue engineering. An embryonal carcinoma cell line, P19.CL6, was cultured in AFS, and the expression and phosphorylation of a gap-junction protein, connexin 43 (Cx43), during cell proliferation and differentiation was examined. We show that treatment with dimethyl sulfoxide appears to induce a change in the isoform composition of Cx43 under the control condition, but not in AFS. We also show that serum starvation induces the phosphorylation of Ser 368 of Cx43 only in functionally mature cells.

Source: Key engineering materials

DOI: 10.4028/www.scientific.net/kem.696.230

Authors: Nobuyuki Kanzawa, Hiroki Takano, Kei Yasuda, Masahiro Takahara, Mamoru Aizawa

Institutions: Sophia University, Meiji University

Language: English

Topic: Connexins and lens biology

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Funder: Japan Society for the Promotion of Science