Work

mCSM-AB: a web server for predicting antibody–antigen affinity changes upon mutation with graph-based signatures

Year: 2016

Type: article

Abstract: Computational methods have traditionally struggled to predict the effect of mutations in antibody-antigen complexes on binding affinity. This has limited their usefulness during antibody engineering and development, and their ability to predict biologically relevant escape mutations. Here we present mCSM-AB, a user-friendly web server for accurately predicting antibody-antigen affinity changes upon mutation which relies on graph-based signatures. We show that mCSM-AB performs better than comparable methods that have been previously used for antibody engineering. mCSM-AB web server is available at http://structure.bioc.cam.ac.uk/mcsm_ab.

Source: Nucleic Acids Research

DOI: 10.1093/nar/gkw458

Authors: Douglas E.V. Pires, David B. Ascher

Institutions: Fiocruz Minas, Fundação Oswaldo Cruz, The University of Melbourne, University of Cambridge

Language: English

Topic: vaccines and immunoinformatics approaches

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,372