Work

Electrochemical Assay of GSTP1-related DNA Sequence for Prostate Cancer Screening

Year: 2012

Type: article

Abstract: A label free detection scheme is developed for a short GTSP-1 (Hypermethylation of glutathione-S-transferase p gene) related DNA sequence (27 mer) based on modified PolyPyrrole (PPy) films deposited at a microelectrode surface. The positively charged PolyPyrrole films are modified by addition of negative charge through phosphoric acid groups of complementary strands to PPy-pTPTC3-PO 3 H 2 -Mg 2+ / probe DNA to create an electrostatic barrier for the incoming anions that influences the kinetics, as noted by cyclic voltammetry (CV). The hypermethylated sequences of GSTP-1 gene are prevalent in prostate cancer patients and this DNA hybridization can be diagnosed from changes in the cyclic voltammogram shape using this assay technique, which will be useful for early detection of the cancer.

Source: The Electrochemical Society Interface

DOI: 10.1149/2.f05123-4if

Authors: A. H. Saheb, M. Leon, M. Josowicz

Institutions: Albany State University, Emory University, Georgia Institute of Technology

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze