Detection of hepatitis A virus RNA with an improved loop-mediated isothermal amplification assay
Year: 2025
Type: article
Abstract: Hepatitis A virus (HAV) is one of the pathogens responsible for acute viral hepatitis and human foodborne diseases, necessitating an urgent medical need for simple and rapid techniques to detect this virus. A novel improved loop-mediated isothermal amplification (LAMP) assay based on the addition of an acceleration primer was developed for hepatitis A virus nucleotide acid detection. Precision and reproducibility analysis revealed high stability and reliability, with a CV range from 0.51 to 0.93%. Comparison between the improved and conventional LAMP assays revealed that in addition to its rapidity and simplicity of use, the former was more sensitive with a detection limit of 5 TCID50/mL. The novel detection method displayed 100% consistency with the TaqMan real-time PCR assay when applied to seven clinical specimens collected from patients with confirmed acute HAV infection. The improved LAMP assay has considerable advantages of rapidity and sensitivity over the conventional assay. Thus, this novel technique is widely applicable as a simple diagnostic tool in the clinical field as well as for the surveillance and investigation of the infectious disease in developing countries where HAV is endemic.
Source: Community Acquired Infection
Authors: Qiudong Su, Feng Qiu, Feng Wang, Shuang Zhang, Ke Xu
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W2405374683