OMIP‐032: Two multi‐color immunophenotyping panels for assessing the innate and adaptive immune cells in the mouse mammary gland
Year: 2016
Type: article
Abstract: A multi-color antibody panel was designed and optimized to identify and characterize 10 leukocyte subpopulations from both the innate and adaptive arms of the immune system.Markers to detect B-cells (CD45.2 1 CD19 1 ), T-cells (CD45.2 1 TCRb 1 ), natural killer cells (CD45.2 1 TCRb 2 CD49b 1 NKp46 1 ), and myeloid cells (CD45.2 1 CD11b 1 ) were employed.Myeloid cells were further differentiated as dendritic cells (CD45.2 1 CD11b 1 CD11c 1 MHCII 1 ), neutrophils (CD45.2 1 CD11b 1 Ly6G 1 ), macrophages (CD45.2 1 CD11b 1 Ly6G 2 Ly6C low ), and monocytes (CD45.2 1 CD11b 1 Ly6G 2 Ly6C high ).T-cell populations were sub-divided into T-helper cells (CD45.2 1 TCRb 1 CD8 2 CD4 1 ), and cytotoxic T-cells (CD45.2 1 TCRb 1 CD4 2 CD8 1 ).T-cell memory/effector status was determined using CD62L and CD44 to distinguish between effector (CD44 1 CD62L 2 ), memory (CD44 1 CD62L 1 ) and na€ ıve (CD44 2 CD62L 1 ) T cells.This panel was established for the analysis of collagenase digested mouse (Balb/ C) mammary gland, spleen and tumor samples, as well as RBC-lysed whole blood (Table 1).
Source: Cytometry Part A
DOI: 10.1002/cyto.a.22867
Authors: Ashleigh Unsworth, Robin Anderson, Nicole Haynes, Kara Britt
Institutions: Peter MacCallum Cancer Centre, The University of Melbourne
Language: English
Topic: T-cell and B-cell Immunology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2405539620