Work

Biosynthesis of Cytidine Diphosphate Diglyceride by Enzyme Preparations from Cauliflower

Year: 1970

Type: article

Abstract: The activity of the enzyme responsible for the synthesis of cytidine diphosphate diglyceride has been found in the 20,000g pellet of a cauliflower (Brassica oleracea) inflorescence homogenate. Greater enzyme activity was found in the pellet after treatment with 0.02% Triton X-100, or after freezing and thawing. Activity was greater when fluoride was included in the reaction mixture. The enzyme has a pH optimum of 5.6, an optimal Mn(2+) concentration of 1mm and an optimal Mg(2+) concentration of 30 mm. At the optimal concentration, Mn(2+) was 4 times better than Mg(2+). The utilization of biosynthesized cytidine diphosphate diglyceride can be enhanced by addition of inositol, thereby causing the formation of phosphatidyl inositol.

Source: PLANT PHYSIOLOGY

DOI: 10.1104/pp.45.6.719

Authors: S. Sumida, J. B. Mudd

Institutions: University of California, Riverside, Statewide Air Pollution Research Center

Language: English

Topic: Lipid metabolism and biosynthesis

Subfield: Biochemistry

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W1975635001

FWCI: 0.798

Cites: 12

Cited by: 19

Related to: 10