Scaffold Composition and Architecture Critically Regulate Extracellular Matrix Synthesis by Cardiomyocytes
Year: 2013
Type: article
Abstract: Heart failure accounts for over 5 million cases in the U.S. A major onset of this is myocardial infarction, which causes the myocardium to loose cardiomyocytes and transform into a scar tissue.Given that the adult infarcted cardiac tissue has a limited ability to regenerate, alternative methods to restore the damaged area need to be developed.The goal of these approaches is to design an optimal scaffold that can retain and deliver cardiomyocytes at the site of damaged myocardium.This tissue engineering approach would allow cardiac reconstruction by replacing the lost cardiomyocytes, delivering the required biomolecules, as well as remodeling the extracellular matrix (ECM).In this study we investigate the effects of a variety of ECM substrates on the attachment, survival and ECM production by cardiomyocytes.We cultured rat cardiomyocytes for 21 days in eleven different substrates, including nanofiber coated plates and 3D hydrogels.Cell attachment and survival rates were analyzed both quantitatively and qualitatively.ELISA and fluorometric assays were performed to quantify the synthesis and release of ECM protein molecules by the cells under various culture conditions.The matrix protein deposition was also qualitatively analyzed using immunofluorescence staining and imaging.Finally, the production of MMPs-2, 9 and CHAPTER I
Author: Arsela Gishto
Institution: Cleveland State University
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2407209187