Work

Phage as a Template to Grow Bone Mineral Nanocrystals

Year: 2013

Type: book-chapter

Abstract: Phage display is a biotechnique that fuses functional peptides on the outer surface of filamentous phage by inserting DNA encoding the peptides into the genes of its coat proteins. The resultant peptide-displayed phage particles have been widely used as biotemplates for the synthesis of functional hybrid nanomaterials. Here, we describe the bioengineering of M13 filamentous phage to surface-display bone mineral (hydroxyapatite (HAP))-nucleating peptides derived from dentin matrix protein-1 and using the engineered phage as a biotemplate to grow HAP nanocrystals.

Source: Methods in molecular biology

DOI: 10.1007/978-1-62703-751-8_10

Authors: Binrui Cao, Hong Xu, Chuanbin Mao

Institution: University of Oklahoma

Language: English

Topic: Bone Tissue Engineering Materials

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: green

Funders: National Heart, Lung, and Blood Institute, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Biomedical Imaging and Bioengineering

Awards: Tubulin-Binding Upconversion Nanoparticles for Breast-Cancer Imaging and Therapy, Bone-seeking and cell-targeting non-viral vectors for BMP-2 gene delivery, Phage-inspired nanoparticles with genetically tunable target-specificity, Phage-Mimetic Nanorods for Targeted Breast Cancer Treatment