Year: 2011
Type: article
Abstract: Post-translational modification of histones and DNA methylation are important components of chromatin-level control of genome activity in eukaryotes. However, principles governing the combinatorial association of chromatin marks along the genome remain poorly understood. Here, we have generated epigenomic maps for eight histone modifications (H3K4me2 and 3, H3K27me1 and 2, H3K36me3, H3K56ac, H4K20me1 and H2Bub) in the model plant Arabidopsis and we have combined these maps with others, produced under identical conditions, for H3K9me2, H3K9me3, H3K27me3 and DNA methylation. Integrative analysis indicates that these 12 chromatin marks, which collectively cover ∼90% of the genome, are present at any given position in a very limited number of combinations. Moreover, we show that the distribution of the 12 marks along the genomic sequence defines four main chromatin states, which preferentially index active genes, repressed genes, silent repeat elements and intergenic regions. Given the compact nature of the Arabidopsis genome, these four indexing states typically translate into short chromatin domains interspersed with each other. This first combinatorial view of the Arabidopsis epigenome points to simple principles of organization as in metazoans and provides a framework for further studies of chromatin-based regulatory mechanisms in plants.
Source: The EMBO Journal
Authors: François Roudier, Ikhlak Ahmed, Caroline Bérard, Alexis Sarazin, Tristan Mary‐Huard, Sandra Cortijo, Daniel Bouyer, Erwann Caillieux, Evelyne Duvernois‐Berthet, Liza Al‐Shikhley, Laurène Giraut, Barbara Després, Stéphanie Drevensek, Frédy Barneche, Sandra Dèrozier, Véronique Brunaud, Sébastien Aubourg, Arp Schnittger, Chris Bowler, Marie‐Laure Martin‐Magniette, Stéphane Robin, Michel Caboche, Vincent Colot
Institutions: Centre National de la Recherche Scientifique, Inserm, Institut de Biologie de l'École Normale Supérieure, AgroParisTech, Mathématiques et Informatique Appliquées, Institut National de la Recherche Agronomique, Institut de Biologie Moléculaire des Plantes, Université de Strasbourg, Institut Jean-Pierre Bourgin, Université d'Évry Val-d'Essonne
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $7,990
OpenAlex ID: W1851394852