MiR-146a activates WAVE2 expression and enhances phagocytosis in lipopolysaccharide-stimulated RAW264.7 macrophages.
Year: 2015
Type: article
Abstract: MiR-146a has been shown to play a critical role in cell immunity and phagocytosis, processes that require rearrangement of the cytoskeleton. However, the detailed mechanism by which miR-146a regulates these events remains elusive. Here, we used luciferase reporter and protein assays to show that the cytoskeleton-regulatingprotein verprolin-homologous protein 2 (WAVE2), is a direct target of miR-146a. MiR-146a overexpression resulted in a decrease in WAVE2 protein expression under endotoxin-free culture conditions. Unexpectedly, however, miR-146a activated rather than repressed the expression of WAVE2 in macrophage RAW264.7 cells when cultured continuously in the presence of endotoxin. Furthermore, we demonstrated that miR-146a induced WAVE2 expression and enhanced phagocytosis in lipopolysaccharide-stimulated RAW264.7 macrophages. Our study suggests that lipopolysaccharide- induced miR146a indirectly activates WAVE2 expression; thus, facilitating cytoskeletal reorganization and phagocytosis in lipopolysaccharide-stimulated macrophages.
Source: Europe PMC (PubMed Central)
Authors: Zhongwei Cao, Qunyan Yao, Shuncai Zhang
Institutions: Fudan University, Zhongshan Hospital
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2409174259