Year: 1982
Type: article
Abstract: Beside diphtheria-and tetanus toxoids, the improved DPT vaccine contains formalinized, alum-precipitated filamentous-and leukocytosis-promoting hemagglutinins from Bordetella pertussis in stead of killed B. pertussis in conventional DPT vaccine.In this paper, the both vaccines were compared on production of IgE antibodies.Sera from rats were tested homocytotropic antibodies, which were comparable to human-IgE antibodies, against diphtheria-and tetanus toxoids by passive cutaneous anaphylaxis (PCA) reaction in rats.In the sera from 27 rats on 16th day after immunization with conventional vaccine, IgE antibodies were detected in 78% against diphtheria toxoid and in 19% against tetanus toxoid. The mean of the antibody titers against diphtheria- and tetanus toxoids were 1: 32 and 1: 2, respectively.In the sera from 27 rats on 16th day after immunization with improved vaccine, IgE antibodies were less detected and the means of the antibody titers against diphtheria-and tetanus toxoids were significantly lower than those immunized with conventional vaccine; IgE antibodies were detected in 41% against diphtheria toxoid and in 7% against tetanus toxoid. The mean of the antibody titers was 1: 2 against diphtheria toxoid and 1: 1 against tetanus toxoid.We suppose that the significantly less production of IgE antibodies in rats immunized with improved DPT vaccine might be due to lower concentration of leukocytosis-promoting factor (LPF) of B. pertussis which has adjuvant effect on production of IgE antibody; the concentration of LPF in improved vaccine is less than 10% of conventional vaccine.On the other hand, the both vaccines elevated neutralizing antibody titers against diphtheria-and tetanus toxins to the level of complete protection from the diseases.
Source: Kansenshogaku zasshi
Authors: Yuichi MACHIDA, Nobuhiro FUKAZAWA, Giichi KANAZAWA
Institution: Kiryu Kosei General Hospital
Language: English
Subfield: Microbiology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2409378308