Work

Genetically encoded optical activation of DNA recombination in human cells

Year: 2016

Type: article

Abstract: We developed two tightly regulated, light-activated Cre recombinase enzymes through site-specific incorporation of two genetically-encoded photocaged amino acids in human cells. Excellent optical off to on switching of DNA recombination was achieved. Furthermore, we demonstrated precise spatial control of Cre recombinase through patterned illumination.

Source: Chemical Communications

DOI: 10.1039/c6cc03934k

Authors: J. Luo, E. Arbely, J. Zhang, C. Chou, R. Uprety, J. W. Chin, A. Deiters

Institutions: University of Pittsburgh, Ben-Gurion University of the Negev, MRC Laboratory of Molecular Biology, Medical Research Council, North Carolina State University

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

OpenAlex ID: W2410873994

FWCI: 2.709

Cites: 39

Cited by: 47

Related to: 10