Work

Comparative Analysis of Eukaryotic Cell-Free Expression Systems

Year: 2015

Type: article

Abstract: Cell-free protein synthesis (CFPS) allows researchers to rapidly generate functional proteins independent of cell culture. Although advances in eukaryotic lysates have increased the amount of protein that can be produced, the nuances of different translation systems lead to variability in protein production. To help overcome this problem, we have compared the relative yield and template requirements for three commonly used commercial cell-free translation systems: wheat germ extract (WGE), rabbit reticulocyte lysate (RRL), and HeLa cell lysate (HCL). Our results provide a general guide for researchers interested in using cell-free translation to generate recombinant protein for biomedical applications.

Source: BioTechniques

DOI: 10.2144/000114327

Authors: Emily M. Hartsough, Pankti Shah, Andrew C. Larsen, John C. Chaput

Institution: Arizona State University

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,415

Funders: National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases