The Use of Functional Tissue Engineering and Mesenchymal Stem Cell Seeded Constructs for Patellar Tendon Repair
Year: 2005
Type: article
Abstract: The objective of this study was to test the governing hypothesis that implanting mesenchymal stem cell (MSC)-seeded collagen gel and collagen sponge scaffolds into surgically induced rabbit patellar tendon (PT) defects will improve the repair tissues' structural and material properties as well as histological appearance.We evaluated the effects of MSC-construct seeding concentration and collagen concentration on the in vivo repair quality after defect injury to the rabbit patellar tendon.In addition, we investigated the effects of adding a collagen sponge scaffold and mechanical stimulation using a silicone dish system on the in vivo repair of the rabbit patellar tendon.The effects of those treatments were assessed using biomechanical and immunohistochemical assays at 12 weeks post surgery.No significant differences were found in the biomechanical properties of the cellgel repairs with different MCS and collagen concentrations.The maximum force and linear stiffness for these repairs was 30% of normal central PT and no ectopic bone was found in any repair site.In a subsequent study, different collagen scaffolds (fibers, films, and sponges) were evaluated in vitro.We found that constructs prepared with cells, collagen gel and collagen sponge exhibited the greatest cell viability, penetration and mechanical integrity after 14 days in culture.In an attempt to increase the stiffness of the repairs, a type I collagen sponge was incorporated into the cell-gel constructs.The cellgel-sponge repairs averaged 75% of the normal tendon linear stiffness and 60% of the normal tendon maximum force.In the next study, cell-gel-sponge constructs were v John West, at School of Medicine in Wright State University Dayton, deserves a very special mention for not only helping and teaching me all about mechanical testing and data collection but more importantly for the long and enthusiastic discussions we have had in interpreting the exciting data from this study.Similarly, special thanks to Cindi Gooch for helping and teaching me all about cell culture work, and helping during surgery.
Author: Laura Natalia Juncosa-Melvin
Institution: University of Cincinnati
Language: English
Subfield: Orthopedics and Sports Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2413884694