E-cadherin maintains the activity of neural stem cells and inhibits the migration.
Year: 2015
Type: article
Abstract: Cadherins are important adhesion molecules that mediate adhesions and communications between cells. These molecules participate in the formation and maintenance of multicellular organisms including the stem cells. E-cadherin is one of the classic cadherins which is reported to be essential for the survival and self-renewal of embryonic stem cells. Moreover, it could induce cell proliferation inhibitory signaling to regulate cell proliferation. In our study, we over-expressed and silenced E-cadherin in NSCs by lentiviral ways. Transgenic cells were confirmed by both quantitative RT-PCR and western blot. Results of MTT assay showed that over-expression of E-cadherin could enhance the cell activity. Furthermore, we performed Transwell chamber assay to analyze its role in regulation of cell migration. The results showed that the migration percent of over-expression cells was lower than control. Our results indicated that E-caherin would maintain the stemness of NSCs and reduce cells migration.
Source: PubMed
Authors: Dong Chen, Zhong Wu, Lin-Jie Luo, Xiang Huang, Wei-Qiang Qian, Hua Wang, Shao-Hua Li, Jie Liu
Institutions: Tongji University, Shanghai Tenth People's Hospital
Language: English
Subfield: Developmental Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2414514823