Occurrence of the enzymes effecting the conversion of acetyl CoA to squalene in homogenates of hog aorta
Year: 1973
Type: article
Abstract: Homogenates and subcellular fractions of the intimamedia of hog aorta have been prepared and examined for the presence of the enzymes catalyzing the conversion of acetyl CoA to squalene. Enzyme activities effecting the conversion of acetyl CoA to 3-hydroxy-3-methylglutarate (HMG); HMG CoA to mevalonic acid; mevalonic acid to 5-phosphomevalonic acid, 5-pyrophosphomevalonic acid, and isopentenyl pyrophosphate; isopentenyl pyrophosphate to farnesyl pyrophosphate; and farnesyl pyrophosphate to squalene have been demonstrated in these homogenates. The overall conversion of mevalonate to squalene has also been demonstrated with recombined fractions of hog aorta homogenates. Data are also presented that suggest that phosphatases present in the crude homogenates act to cleave farnesyl pyrophosphate to farnesol, and phospho- and pyrophosphomevalonate to mevalonate.
Source: Journal of Lipid Research
Institution: University of Wisconsin–Madison
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
OpenAlex ID: W2414714327