Work

Preparation and Analysis of Uniquely Positioned Mononucleosomes

Year: 2009

Type: book-chapter

Abstract: Short DNA fragments containing single, uniquely positioned nucleosome cores have been extensively employed as simple model experimental systems for analysis of many intranuclear processes, including binding of proteins to nucleosomes, transcription, DNA repair and ATP-dependent chromatin remodeling. In many cases such simple model templates faithfully recapitulate numerous important aspects of these processes. Here we describe several recently developed procedures for obtaining and analysis of mononucleosomes that are uniquely positioned on 150-600 bp DNA fragments.

Source: Methods in molecular biology

DOI: 10.1007/978-1-59745-190-1_8

Authors: Daria A. Gaykalova, Olga I. Kulaeva, Vladimir A. Bondarenko, Vasily M. Studitsky

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute, National Institute of General Medical Sciences

Awards: G1035068280, G1165598027, G1667130490

OpenAlex ID: W2416036156

FWCI: 1.188

Cites: 37

Cited by: 55

Related to: 10