Work

3� Rapid Amplification of cDNA Ends (3� RACE) Using Arabidopsis Samples

Year: 2015

Type: article

Abstract: reproductive tissues, can be applied to characterize RNA species from other organs (leaf, root) and/or other plant species. We provide a detailed protocol of our 3' RACE procedure comprising four major parts: Total RNA extraction, RNA amount determination and quality control, the RACE procedure itself, and isolation of the resulting RACE products for cloning and sequencing.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.1604

Authors: Encarnaci�n Rodr�guez-Cazorla, Alfonso And�jar, Juan Ripoll, Lindsay Bailey, Antonio Mart�nez-Laborda, Martin Yanofsky, Antonio Vera

Institutions: Universitat de Miguel Hernández d'Elx, University of California San Diego

Language: English

Topic: Molecular Biology Techniques and Applications

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Award: A novel genetic network controlling meristem initiation and stem cell patterning

OpenAlex ID: W2417282561

FWCI: 0.145

Cites: 6

Cited by: 7

Related to: 10