Year: 2016
Type: dissertation
Abstract: In the last 15 years a number of studies have sought to identify markers of tumour endothelium. However, studies have yet to identify non-protein markers of the tumour vasculature. This thesis provides novel contributions in this area by using RNA sequencing (RNAseq) to probe the transcriptome of tumour endothelial cells (TEC) and healthy tissue associated endothelial cells (HEC). The ensuing bioinformatic analysis revealed many differentially expressed short nuclear RNA (snoRNA) and long intergenic ncRNA (lincRNA) molecules. Some of which were confirmed as being specifically expressed in (and differentially expressed between) TEC and HEC using quantitative PCR (qPCR). One of these molecules was also functionally explored in vitro and is the first ncRNA molecule to be identified as both specific to the endothelium and to be functional in pathological angiogenesis.
Author: Klarke M. Sample
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2417658205