Work

In Vivo chiro-lnositol Metabolism in the Rat: A Defect in chiro-Inositol Synthesis from myo-lnositol and an Increased Incorporation of chiro-[3H]inositol into Phospholipid in the Goto-Kakizaki (G.K.) Rat

Year: 1998

Type: article

Abstract: We report our comparative studies of myo- and chiro-[3H]inositol distribution in control nondiabetic Wistar and spontaneous nonobese insulin-resistant Type II diabetic G.K. rat tissues following 78-h labeling period. From various tissue extracts, free inositols, inositol phosphates, and inositol phospholipids were isolated and analyzed. Our findings demonstrate (1) a significant difference in the metabolism of myo- and chiro-inositol in normal Wistar and G.K. rats, (2) a severe defect in conversion of myo-[3H]inositol to chiro-[3H]inositol at the inositol phospholipid level of insulin-sensitive tissues of the G.K. rat, (3) an increased incorporation of myo-[3H]inositol into purified inositol phospholipids of the G.K. rat consistent with a decreased conversion to chiro-[3H]inositol, (4) the presumed presence of a pathway which incorporates chiro-[3H]inositol into inositol phospholipids, and which is overactive in the G.K. rat compared to the Wistar rat, and (5) no or minimal conversion of chiro-[3H]inositol to myo-[3H]inositol.

Source: Molecules and Cells

DOI: 10.1016/s1016-8478(23)13428-5

Authors: Yunbae Pak, Yonggeun Hong, Seokyong Kim, Tom Piccariello, Robert V. Farese, Joseph Larner

Institutions: Gyeongsang National University, University of Virginia, University of South Florida, James A. Haley Veterans' Hospital

Language: English

Topic: Lipid metabolism and biosynthesis

Subfield: Biochemistry

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $2,500

Funder: National Institute of Diabetes and Digestive and Kidney Diseases