Year: 2015
Type: article
Abstract: Human pluripotent stem cells (hPSCs) can self-renew or differentiate to diverse cell types, thus providing a platform for basic and clinical applications. However, pluripotent stem cell populations are heterogeneous and functional properties at the single cell level are poorly documented leading to inefficiencies in differentiation and concerns regarding reproducibility and safety. Here, we use non-invasive time-lapse imaging to continuously examine hPSC maintenance and differentiation and to predict cell viability and fate. We document dynamic behaviors and social interactions that prospectively distinguish hPSC survival, self-renewal, and differentiation. Results highlight the molecular role of E-cadherin not only for cell-cell contact but also for clonal propagation of hPSCs. Results indicate that use of continuous time-lapse imaging can distinguish cellular heterogeneity with respect to pluripotency as well as a subset of karyotypic abnormalities whose dynamic properties were monitored.
Source: Scientific Reports
DOI: 10.1038/srep14209
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,690
Funders: California Institute for Regenerative Medicine, National University of Singapore, National Institutes of Health, National Heart, Lung, and Blood Institute, National Institute of Biomedical Imaging and Bioengineering, Eunice Kennedy Shriver National Institute of Child Health and Human Development
Awards: Predoctoral Training in Biomedical Imaging at Stanford University, Stanford University Center for Reproductive and Stem Cell biology, Basic and Translational Research iPSC-based hematologic and vascular therapies, Stanford University Center for Reproductive and Stem Cell biology, Autologous iPSC Therapy for Urinary Incontinence, G9000249620
OpenAlex ID: W2427299411