Work

Real-time fluorescence imaging with 20 nm axial resolution

Year: 2015

Type: article

Abstract: Measuring the nanoscale organization of protein structures near the plasma membrane of live cells is challenging, especially when the structure is dynamic. Here we present the development of a two-wavelength total internal reflection fluorescence method capable of real-time imaging of cellular structure height with nanometre resolution. The method employs a protein of interest tagged with two different fluorophores and imaged to obtain the ratio of emission in the two channels. We use this approach to visualize the nanoscale organization of microtubules and endocytosis of the epidermal growth factor receptor.

Source: Nature Communications

DOI: 10.1038/ncomms9307

Authors: Daniel R. Stabley, Thomas Oh, Sanford M. Simon, Alexa L. Mattheyses, Khalid Salaita

Institutions: Emory University, Rockefeller University

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $6,990