Work

Metal ion transport quantified by ICP-MS in intact cells

Year: 2016

Type: article

Abstract: The use of ICP-MS to measure metal ion content in biological tissues offers a highly sensitive means to study metal-dependent physiological processes. Here we describe the application of ICP-MS to measure membrane transport of Rb and K ions by the Na,K-ATPase in mouse skeletal muscles and human red blood cells. The ICP-MS method provides greater precision and statistical power than possible with conventional tracer flux methods. The method is widely applicable to studies of other metal ion transporters and metal-dependent processes in a range of cell types and conditions.

Source: Scientific Reports

DOI: 10.1038/srep20551

Authors: Julio A. Landero Figueroa, Cory A. Stiner, Tatiana L. Radzyukevich, Judith A. Heiny

Institution: University of Cincinnati

Language: English

Topic: Analytical chemistry methods development

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $2,690

Funders: National Institutes of Health, National Institute of Arthritis and Musculoskeletal and Skin Diseases