Work

Determination of the Kinetics and Thermodynamics of Ligand Binding to a Specific Inactive Conformation in Protein Kinases

Year: 2012

Type: book-chapter

Abstract: Recent interest in inactive kinase conformations has generated the need to develop new biochemical tools to study them. Here, we describe the use of a fluorescent probe that selectively and potently binds to a specific inactive conformation of protein kinases. This allows for the thermodynamics and kinetics of ligand binding to be determined.

Source: Methods in molecular biology

DOI: 10.1007/978-1-62703-008-3_12

Authors: Sanjay B. Hari, Pratistha Ranjitkar, Dustin J. Maly

Institution: University of Washington

Language: English

Topic: Protein Kinase Regulation and GTPase Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Award: G3527409994