Determining the Effect of Substitutions at Alanine 47 in Synechococcus Pcc6301 Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (RUBISCO)
Year: 2006
Type: article
Abstract: Determining the Effect of Substitutions at Alanine 47 in Synechococcus PCC6301 Ribulose-1,5-bisphosphate Carboxylase/Oxygenase (RubisCO).Mutant screening and genetic selection in various organisms have shown that residues far from the active site of ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) can influence catalytic efficiency and CO 2 /O 2 specificity.Because RubisCO catalyzes the rate-limiting step of photosynthesis, further study of these sites distant from the primary reaction center may provide the necessary information for engineering an increase in primary productivity of crop plants.In a previously described system of random mutagenesis and bioselection (Smith, 2002), the RubisCO genes from the cyanobacterium Synechococcus PCC6301, were randomly mutated and introduced into the photosynthetic bacterium Rhodobacter capsulatus.An A47T substitution resulted in a very modest loss of specific activity.However, this mutant was not functional relative to the wild-type when complementation was performed.Since mutants with even lower catalytic activity were able to complement a RubisCO deletion strain Most of all, I am grateful to my parents, Stephen and Elaine Salyer, along with my wife, Lauren Salyer, for their unending enthusiasm and support over the past two years.They have never stopped believing in me.This research is dedicated to them.
Author: Christopher R. Salyer
Institution: Wright State University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2439367313