Adenovirus-Mediated Gene Transfection in the Isolated Lymphatic Vessels
Year: 2011
Type: book-chapter
Abstract: The authors describe technical details of experimental protocol of gene transfection in isolated rat mesenteric lymphatic vessels (MLVs). Authors also refer to the recent publication in Microcirculation, which provides wide set of experimental evidences obtained from confocal microscopic imaging and isolated vessels functional tests, which confirmed a successful achievement of the following goals. (1) Optimization of the experimental conditions to maintain the isolated "normal" rat mesenteric vessels in culture for sufficiently long periods of time to permit effective knockdown or overexpression of selected proteins/genes. (2) Development of the effective transfection protocols for lymphatic muscle and/or endothelial cells in intact isolated rat MLVs without nonspecific impairment on lymphatic contractile function due to the transfection protocol per se.
Source: Methods in molecular biology
Institutions: Texas A&M Health Science Center, Texas A&M University
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: green
Awards: Mechanisms of the Age-related Alterations in Lymphatic Pumping, G177108904, Influences of Lymph Flow on the Lymphatic Pump, G3033180827, Molecular Mechanisms of Lymphatic Muscle Contraction, Regulatory Mechanisms in Lymphatic Muscle Contraction, G3845515832, Physiological Basis of Vascular Contractility, Ionic Mechanisms of Stretch-Activation in Muscular Lymphatics, G7096095146, Cellular Mechanisms of Lymphatic Muscle Contractility, G945664642
OpenAlex ID: W2440534313