Work

Facile Removal of Leader Peptides from Lanthipeptides by Incorporation of a Hydroxy Acid

Year: 2015

Type: article

Abstract: The biosynthesis of ribosomally synthesized and post-translationally modified peptide (RiPP) natural products typically involves a precursor peptide which contains a leader peptide that is important for the modification process, and that is removed in the final step by a protease. Genome mining efforts for new RiPPs are often hampered by the lack of a general method to remove the leader peptides. We describe here the incorporation of hydroxy acids into the precursor peptides in E. coli which results in connection of the leader peptide via an ester linkage that is readily cleaved by simple hydrolysis. We demonstrate the method for two lantibiotics, lacticin 481 and nukacin ISK-1.

Source: Journal of the American Chemical Society

DOI: 10.1021/jacs.5b04681

Authors: Noah A. Bindman, Silvia C. Bobeica, Wenshe R. Liu, Wilfred A. van der Donk

Institutions: Howard Hughes Medical Institute, University of Illinois Urbana-Champaign, Texas A&M University

Language: English

Topic: Microbial Natural Products and Biosynthesis

Subfield: Pharmacology

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: Howard Hughes Medical Institute, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Environmental Health Sciences