Work

CpG methylation of an endogenous retroviral enhancer inhibits transcription factor binding and activity.

Year: 1991

Type: article

Abstract: The endogenous retrovirus, intracisternal A-particle (IAP), is expressed at unique stages during murine embryogenesis and is also activated during the in vitro differentiation of F9 cells. We have examined the DNA elements and protein factors that control IAP expression during F9 differentiation. In the present study an IAP upstream enhancer (IUE) is identified by transient transfection assays and found to be active in both undifferentiated and differentiated cells. Further analyses reveal that a ubiquitous 65 kDa protein factor, the IUE binding protein (IUEB), binds with the IUE. Site-specific methylation within the IUEB binding site strongly inhibits both IUEB binding and IUE transcriptional activity, suggesting that methylation may regulate IUE function and IAP expression.

Source: Europe PMC (PubMed Central)

Authors: B T Lamb, K Satyamoorthy, L Li, D Solter, C C Howe

Institution: The Wistar Institute

Language: English

Topic: Epigenetics and DNA Methylation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Cancer Institute, National Institute of General Medical Sciences, Eunice Kennedy Shriver National Institute of Child Health and Human Development

Awards: Training Program in Cell and Molecular Biology, CONTROLLING MECHANISMS IN MAMMALIAN REPRODUCTION, CELL LINEAGE IN DEVELOPMENT, NUCLEO-CYTOPLASMIC INTERACTIONS IN DEVELOPMENT, Consolidated Basic Cancer Research Program

OpenAlex ID: W2461793521

FWCI: 0.779

Cites: 58

Cited by: 24

Related to: 10